by Barnett, Brad P, Ruiz-Cabello, Jesus, Hota, Partha, Ouwerkerk, Ronald, Shamblott, Michael J, Lauzon, Cal, Walczak, Piotr, Gilson, Wesley D, Chacko, Vadappuram P, Kraitchman, Dara L, Arepally, Aravind and Bulte, Jeff W M
Abstract:
In vivo imaging of engraftment and immunorejection of transplanted islets is critical for further clinical development, with (1)H MR imaging of superparamagnetic iron oxide-labeled cells being the current premier modality. Using perfluorocarbon nanoparticles, we present here a strategy for non-invasive imaging of cells using other modalities. To this end, human cadaveric islets were labeled with rhodamine-perfluorooctylbromide (PFOB) nanoparticles, rhodamine-perfluoropolyether (PFPE) nanoparticles or Feridex as control and tested in vitro for cell viability and c-peptide secretion for 1 week. (19)F MRI, computed tomography (CT) and ultrasound (US) imaging was performed on labeled cell phantoms and on cells following transplantation beneath the kidney capsule of mice and rabbits. PFOB and PFPE-labeling did not reduce human islet viability or glucose responsiveness as compared with unlabeled cells or SPIO-labeled cells. PFOB- and PFPE-labeled islets were effectively fluorinated for visualization by (19)F MRI. PFOB-labeled islets were acoustically reflective for detection by US imaging and became sufficiently brominated to become radiopaque allowing visualization with CT. Thus, perfluorocarbon nanoparticles are multimodal cellular contrast agents that may find applications in real-time targeted delivery and imaging of transplanted human islets or other cells in a clinically applicable manner using MRI, US or CT imaging.
Reference:
Use of perfluorocarbon nanoparticles for non-invasive multimodal cell tracking of human pancreatic islets. (Barnett, Brad P, Ruiz-Cabello, Jesus, Hota, Partha, Ouwerkerk, Ronald, Shamblott, Michael J, Lauzon, Cal, Walczak, Piotr, Gilson, Wesley D, Chacko, Vadappuram P, Kraitchman, Dara L, Arepally, Aravind and Bulte, Jeff W M), In Contrast media & molecular imaging, John Wiley & Sons, Ltd., volume 6, 2011.
Bibtex Entry:
@article{Barnett:2011btg,
author = {Barnett, Brad P and Ruiz-Cabello, Jesus and Hota, Partha and Ouwerkerk, Ronald and Shamblott, Michael J and Lauzon, Cal and Walczak, Piotr and Gilson, Wesley D and Chacko, Vadappuram P and Kraitchman, Dara L and Arepally, Aravind and Bulte, Jeff W M},
title = {{Use of perfluorocarbon nanoparticles for non-invasive multimodal cell tracking of human pancreatic islets.}},
journal = {Contrast media {&} molecular imaging},
year = {2011},
volume = {6},
number = {4},
pages = {251--259},
month = jul,
publisher = {John Wiley {&} Sons, Ltd.},
affiliation = {Department of Radiology and Radiological Science, Division of MR Research, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.},
doi = {10.1002/cmmi.424},
pmid = {21861285},
pmcid = {PMC3160722},
language = {English},
rating = {0},
date-added = {2018-03-16T12:56:39GMT},
date-modified = {2018-04-04T07:59:10GMT},
abstract = {In vivo imaging of engraftment and immunorejection of transplanted islets is critical for further clinical development, with (1)H MR imaging of superparamagnetic iron oxide-labeled cells being the current premier modality. Using perfluorocarbon nanoparticles, we present here a strategy for non-invasive imaging of cells using other modalities. To this end, human cadaveric islets were labeled with rhodamine-perfluorooctylbromide (PFOB) nanoparticles, rhodamine-perfluoropolyether (PFPE) nanoparticles or Feridex as control and tested in vitro for cell viability and c-peptide secretion for 1 week. (19)F MRI, computed tomography (CT) and ultrasound (US) imaging was performed on labeled cell phantoms and on cells following transplantation beneath the kidney capsule of mice and rabbits. PFOB and PFPE-labeling did not reduce human islet viability or glucose responsiveness as compared with unlabeled cells or SPIO-labeled cells. PFOB- and PFPE-labeled islets were effectively fluorinated for visualization by (19)F MRI. PFOB-labeled islets were acoustically reflective for detection by US imaging and became sufficiently brominated to become radiopaque allowing visualization with CT. Thus, perfluorocarbon nanoparticles are multimodal cellular contrast agents that may find applications in real-time targeted delivery and imaging of transplanted human islets or other cells in a clinically applicable manner using MRI, US or CT imaging.},
url = {http://doi.wiley.com/10.1002/cmmi.424},
uri = {url{papers3://publication/doi/10.1002/cmmi.424}}
}