by Callejo, Maria, Mondejar-Parreño, Gema, Barreira, Bianca, Izquierdo-García, José L, Morales-Cano, Daniel, Esquivel-Ruiz, Sergio, Moreno, Laura, Cogolludo, Angel, Duarte, Juan and Perez-Vizcaino, Francisco
Abstract:
We have analysed whether pulmonary arterial hypertension (PAH) alters the rat faecal microbiota. Wistar rats were injected with the VEGF receptor antagonist SU5416 (20 mg/kg s.c.) and followed for 2 weeks kept in hypoxia (10% O2, PAH) or injected with vehicle and kept in normoxia (controls). Faecal samples were obtained and microbiome composition was determined by 16S rRNA gene sequencing and bioinformatic analysis. No effect of PAH on the global microbiome was found ($alpha$- or $beta$-diversity). However, PAH-exposed rats showed gut dysbiosis as indicated by a taxonomy-based analysis. Specifically, PAH rats had a three-fold increase in Firmicutes-to-Bacteroidetes ratio. Within the Firmicutes phylum, there were no large changes in the relative abundance of the bacterial families in PAH. Among Bacteroidetes, all families were less abundant in PAH. A clear separation was observed between the control and PAH clusters based on short chain fatty acid producing bacterial genera. Moreover, acetate was reduced in the serum of PAH rats. In conclusion, faecal microbiota composition is altered as a result of PAH. This misbalanced bacterial ecosystem might in turn play a pathophysiological role in PAH by altering the immunologic, hormonal and metabolic homeostasis.
Reference:
Pulmonary Arterial Hypertension Affects the Rat Gut Microbiome. (Callejo, Maria, Mondejar-Parreño, Gema, Barreira, Bianca, Izquierdo-García, José L, Morales-Cano, Daniel, Esquivel-Ruiz, Sergio, Moreno, Laura, Cogolludo, Angel, Duarte, Juan and Perez-Vizcaino, Francisco), In Scientific Reports, Nature Publishing Group, volume 8, 2018.
Bibtex Entry:
@article{Callejo:2018jm,
author = {Callejo, Maria and Mondejar-Parre{~n}o, Gema and Barreira, Bianca and Izquierdo-Garc{'i}a, Jos{'e} L and Morales-Cano, Daniel and Esquivel-Ruiz, Sergio and Moreno, Laura and Cogolludo, Angel and Duarte, Juan and Perez-Vizcaino, Francisco},
title = {{Pulmonary Arterial Hypertension Affects the Rat Gut Microbiome.}},
journal = {Scientific Reports},
year = {2018},
volume = {8},
number = {1},
pages = {9681--10},
month = jun,
publisher = {Nature Publishing Group},
affiliation = {Departamento de Farmacolog{'i}a y Toxicolog{'i}a. Facultad de Medicina, Universidad Complutense de Madrid, 28040, Madrid, Spain.},
doi = {10.1038/s41598-018-27682-w},
pmid = {29946072},
pmcid = {PMC6018770},
language = {English},
rating = {0},
date-added = {2020-03-17T19:54:27GMT},
date-modified = {2020-07-09T13:27:47GMT},
abstract = {We have analysed whether pulmonary arterial hypertension (PAH) alters the rat faecal microbiota. Wistar rats were injected with the VEGF receptor antagonist SU5416 (20 mg/kg s.c.) and followed for 2 weeks kept in hypoxia (10% O2, PAH) or injected with vehicle and kept in normoxia (controls). Faecal samples were obtained and microbiome composition was determined by 16S rRNA gene sequencing and bioinformatic analysis. No effect of PAH on the global microbiome was found ($alpha$- or $beta$-diversity). However, PAH-exposed rats showed gut dysbiosis as indicated by a taxonomy-based analysis. Specifically, PAH rats had a three-fold increase in Firmicutes-to-Bacteroidetes ratio. Within the Firmicutes phylum, there were no large changes in the relative abundance of the bacterial families in PAH. Among Bacteroidetes, all families were less abundant in PAH. A clear separation was observed between the control and PAH clusters based on short chain fatty acid producing bacterial genera. Moreover, acetate was reduced in the serum of PAH rats. In conclusion, faecal microbiota composition is altered as a result of PAH. This misbalanced bacterial ecosystem might in turn play a pathophysiological role in PAH by altering the immunologic, hormonal and metabolic homeostasis.},
url = {http://www.nature.com/articles/s41598-018-27682-w},
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