by Cogliati, Sara, Herranz, Fernando, Ruiz-Cabello, Jesus and Enríquez, José Antonio
Abstract:
The BlueNative page (BNGE) gel has been the reference technique for studying the electron transport chain organization since it was established 20 years ago. Although the migration of supercomplexes has been demonstrated being real, there are still several concerns about its ability to reveal genuine interactions between respiratory complexes. Moreover, the use of different solubilization conditions generates conflicting interpretations. Here, we thoroughly compare the impact of different digitonin concentrations on the liquid dispersions’ physical properties and correlate with the respiratory complexes’ migration pattern and supercomplexes. Our results demonstrate that digitonin concentration generates liquid dispersions with specific size and variability critical to distinguish between a real association of complexes from being trapped in the same micelle.
Reference:
Digitonin concentration is determinant for mitochondrial supercomplexes analysis by BlueNative page. (Cogliati, Sara, Herranz, Fernando, Ruiz-Cabello, Jesus and Enríquez, José Antonio), In Biochimica et biophysica acta. Bioenergetics, volume 1862, 2021.
Bibtex Entry:
@article{Cogliati:2021dx,
author = {Cogliati, Sara and Herranz, Fernando and Ruiz-Cabello, Jesus and Enr{'i}quez, Jos{'e} Antonio},
title = {{Digitonin concentration is determinant for mitochondrial supercomplexes analysis by BlueNative page.}},
journal = {Biochimica et biophysica acta. Bioenergetics},
year = {2021},
volume = {1862},
number = {1},
pages = {148332},
month = jan,
affiliation = {Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Melchor Fern{'a}ndez Almagro, 3, 28029 Madrid, Spain; Departamento de Biolog{'i}a Molecular, Centro de Biolog{'i}a Molecular Severo Ochoa, Consejo Superior de Investigaciones Cient{'i}ficas-Universidad Aut{'o}noma de Madrid (CSIC-UAM), Madrid, Spain. Electronic address: sara.cogliati@uam.es.},
doi = {10.1016/j.bbabio.2020.148332},
pmid = {33129827},
language = {English},
rating = {0},
date-added = {2021-02-10T07:18:40GMT},
date-modified = {2021-02-10T07:18:42GMT},
abstract = {The BlueNative page (BNGE) gel has been the reference technique for studying the electron transport chain organization since it was established 20~years ago. Although the migration of supercomplexes has been demonstrated being real, there are still several concerns about its ability to reveal genuine interactions between respiratory complexes. Moreover, the use of different solubilization conditions generates conflicting interpretations. Here, we thoroughly compare the impact of different digitonin concentrations on the liquid dispersions' physical properties and correlate with the respiratory complexes' migration pattern and supercomplexes. Our results demonstrate that digitonin concentration generates liquid dispersions with specific size and variability critical to distinguish between a real association of complexes from being trapped in the same micelle.},
url = {https://linkinghub.elsevier.com/retrieve/pii/S0005272820301821},
uri = {url{papers3://publication/doi/10.1016/j.bbabio.2020.148332}}
}