by Cogliati, Sara, Herranz, Fernando, Ruiz-Cabello, Jesús and Enríquez, José Antonio
Abstract:
The BlueNative page (BNGE) gel has been the reference technique for studying the electron transport chain organization since it was established 20 years ago. Although the migration of supercomplexes has been demonstrated being real, there are still several concerns about its ability to reveal genuine interactions between respiratory complexes. Moreover, the use of different solubilization conditions generates conflicting in­ terpretations. Here, we thoroughly compare the impact of different digitonin concentrations on the liquid dis­ persions’ physical properties and correlate with the respiratory complexes’ migration pattern and supercomplexes. Our results demonstrate that digitonin concentration generates liquid dispersions with specific size and variability critical to distinguish between a real association of complexes from being trapped in the same micelle.
Reference:
Digitonin concentration is determinant for mitochondrial supercomplexes analysis by BlueNative page (Cogliati, Sara, Herranz, Fernando, Ruiz-Cabello, Jesús and Enríquez, José Antonio), In Biochimica et Biophysica Acta (BBA) – Bioenergetics, volume 1862, 2021.
Bibtex Entry:
@article{cogliati_digitonin_2021,
	title = {Digitonin concentration is determinant for mitochondrial supercomplexes analysis by {BlueNative} page},
	volume = {1862},
	issn = {00052728},
	url = {https://linkinghub.elsevier.com/retrieve/pii/S0005272820301821},
	doi = {10.1016/j.bbabio.2020.148332},
	abstract = {The BlueNative page (BNGE) gel has been the reference technique for studying the electron transport chain organization since it was established 20 years ago. Although the migration of supercomplexes has been demonstrated being real, there are still several concerns about its ability to reveal genuine interactions between respiratory complexes. Moreover, the use of different solubilization conditions generates conflicting in­ terpretations. Here, we thoroughly compare the impact of different digitonin concentrations on the liquid dis­ persions’ physical properties and correlate with the respiratory complexes’ migration pattern and supercomplexes. Our results demonstrate that digitonin concentration generates liquid dispersions with specific size and variability critical to distinguish between a real association of complexes from being trapped in the same micelle.},
	language = {en},
	number = {1},
	urldate = {2020-11-10},
	journal = {Biochimica et Biophysica Acta (BBA) - Bioenergetics},
	author = {Cogliati, Sara and Herranz, Fernando and Ruiz-Cabello, Jesús and Enríquez, José Antonio},
	month = jan,
	year = {2021},
	pages = {148332},
	file = {Cogliati et al. - 2021 - Digitonin concentration is determinant for mitocho.pdf:/Users/jruizcabello/Zotero/storage/NENZDRN4/Cogliati et al. - 2021 - Digitonin concentration is determinant for mitocho.pdf:application/pdf}
}