by Izidoro, Mario Augusto, Cecconi, Alberto, Panadero, María Isabel, Mateo, Jesus, Godzien, Joanna, Vilchez, Jean Paul, López-Gonzálvez, Ángeles, Ruiz-Cabello, Jesus, Ibáñez, Borja, Barbas, Coral and Rupérez, Francisco J
Abstract:
Balloon catheter endothelial denudation in New Zealand white rabbits fed high cholesterol diet is a validated atherosclerosis model. Well-characterized in terms of atherosclerosis induction and progression, the metabolic changes associated with the atherosclerosis progression remain indeterminate. Non-targeted metabolomics permits to develop such elucidation and allows to evaluate the metabolic consequences of colchicine treatment, an anti-inflammatory drug that could revert these changes. 16 rabbits underwent 18 weeks of atherosclerosis induction by diet and aortic denudation. Thereafter animals were randomly assigned to colchicine treatment or placebo for 18 weeks while on diet. Plasma samples were obtained before randomization and at 36 weeks. Multiplatform (GC/MS, CE/MS, RP-HPLC/MS) metabolomics was applied. Plasma fingerprints were pre-processed, and the resulting matrixes analyzed to unveil differentially expressed features. Different chemical annotation strategies were accomplished for those significant features. We found metabolites associated with either atherosclerosis progression, or colchicine treatment, or both. Atherosclerosis was profoundly associated with an increase in circulating bile acids. Most of the changes associated with sterol metabolism could not be reverted by colchicine treatment. However, the variations in lysine, tryptophan and cysteine metabolism among others, have shown new potential mechanisms of action of the drug, also related to atherosclerosis progression, but not previously described.
Reference:
Plasma Metabolic Signature of Atherosclerosis Progression and Colchicine Treatment in Rabbits. (Izidoro, Mario Augusto, Cecconi, Alberto, Panadero, María Isabel, Mateo, Jesus, Godzien, Joanna, Vilchez, Jean Paul, López-Gonzálvez, Ángeles, Ruiz-Cabello, Jesus, Ibáñez, Borja, Barbas, Coral and Rupérez, Francisco J), In Scientific Reports, Nature Publishing Group, volume 10, 2020.
Bibtex Entry:
@article{Izidoro:2020dka,
author = {Izidoro, Mario Augusto and Cecconi, Alberto and Panadero, Mar{'i}a Isabel and Mateo, Jesus and Godzien, Joanna and Vilchez, Jean Paul and L{'o}pez-Gonz{'a}lvez, {'A}ngeles and Ruiz-Cabello, Jesus and Ib{'a}{~n}ez, Borja and Barbas, Coral and Rup{'e}rez, Francisco J},
title = {{Plasma Metabolic Signature of Atherosclerosis Progression and Colchicine Treatment in Rabbits.}},
journal = {Scientific Reports},
year = {2020},
volume = {10},
number = {1},
pages = {7072--9},
month = apr,
publisher = {Nature Publishing Group},
affiliation = {Centro de Metabol{'o}mica y Bioan{'a}lisis (CEMBIO), Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanizaci{'o}n Montepr{'i}ncipe, Boadilla del Monte, 28660, Madrid, Spain.},
doi = {10.1038/s41598-020-63306-y},
pmid = {32341369},
pmcid = {PMC7184732},
language = {English},
rating = {0},
date-added = {2020-05-13T19:16:08GMT},
date-modified = {2020-10-07T09:51:48GMT},
abstract = {Balloon catheter endothelial denudation in New Zealand white rabbits fed high cholesterol diet is a validated atherosclerosis model. Well-characterized in terms of atherosclerosis induction and progression, the metabolic changes associated with the atherosclerosis progression remain indeterminate. Non-targeted metabolomics permits to develop such elucidation and allows to evaluate the metabolic consequences of colchicine treatment, an anti-inflammatory drug that could revert these changes. 16 rabbits underwent 18 weeks of atherosclerosis induction by diet and aortic denudation. Thereafter animals were randomly assigned to colchicine treatment or placebo for 18 weeks while on diet. Plasma samples were obtained before randomization and at 36 weeks. Multiplatform (GC/MS, CE/MS, RP-HPLC/MS) metabolomics was applied. Plasma fingerprints were pre-processed, and the resulting matrixes analyzed to unveil differentially expressed features. Different chemical annotation strategies were accomplished for those significant features. We found metabolites associated with either atherosclerosis progression, or colchicine treatment, or both. Atherosclerosis was profoundly associated with an increase in circulating bile acids. Most of the changes associated with sterol metabolism could not be reverted by colchicine treatment. However, the variations in lysine, tryptophan and cysteine metabolism among others, have shown new potential mechanisms of action of the drug, also related to atherosclerosis progression, but not previously described.},
url = {http://www.nature.com/articles/s41598-020-63306-y},
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