by Izquierdo-García, José L, Viswanath, Pavithra, Eriksson, Pia, Cai, Larry, Radoul, Marina, Chaumeil, Myriam M, Blough, Michael, Luchman, H Artee, Weiss, Samuel, Cairncross, J Gregory, Phillips, Joanna J, Pieper, Russell O and Ronen, Sabrina M
Abstract:
Mutant isocitrate dehydrogenase 1 (IDH1) catalyzes the production of 2-hydroxyglutarate but also elicits additional metabolic changes. Levels of both glutamate and pyruvate dehydrogenase (PDH) activity have been shown to be affected in U87 glioblastoma cells or normal human astrocyte (NHA) cells expressing mutant IDH1, as compared with cells expressing wild-type IDH1. In this study, we show how these phenomena are linked through the effects of IDH1 mutation, which also reprograms pyruvate metabolism. Reduced PDH activity in U87 glioblastoma and NHA IDH1 mutant cells was associated with relative increases in PDH inhibitory phosphorylation, expression of pyruvate dehydrogenase kinase-3, and levels of hypoxia inducible factor-1$alpha$. PDH activity was monitored in these cells by hyperpolarized (13)C-magnetic resonance spectroscopy ((13)C-MRS), which revealed a reduction in metabolism of hyperpolarized 2-(13)C-pyruvate to 5-(13)C-glutamate, relative to cells expressing wild-type IDH1. (13)C-MRS also revealed a reduction in glucose flux to glutamate in IDH1 mutant cells. Notably, pharmacological activation of PDH by cell exposure to dichloroacetate (DCA) increased production of hyperpolarized 5-(13)C-glutamate in IDH1 mutant cells. Furthermore, DCA treatment also abrogated the clonogenic advantage conferred by IDH1 mutation. Using patient-derived mutant IDH1 neurosphere models, we showed that PDH activity was essential for cell proliferation. Taken together, our results established that the IDH1 mutation induces an MRS-detectable reprogramming of pyruvate metabolism, which is essential for cell proliferation and clonogenicity, with immediate therapeutic implications.
Reference:
IDH1 Mutation Induces Reprogramming of Pyruvate Metabolism. (Izquierdo-García, José L, Viswanath, Pavithra, Eriksson, Pia, Cai, Larry, Radoul, Marina, Chaumeil, Myriam M, Blough, Michael, Luchman, H Artee, Weiss, Samuel, Cairncross, J Gregory, Phillips, Joanna J, Pieper, Russell O and Ronen, Sabrina M), In Cancer Research, American Association for Cancer Research, volume 75, 2015.
Bibtex Entry:
@article{IzquierdoGarcia:2015bda,
author = {Izquierdo-Garc{'i}a, Jos{'e} L and Viswanath, Pavithra and Eriksson, Pia and Cai, Larry and Radoul, Marina and Chaumeil, Myriam M and Blough, Michael and Luchman, H Artee and Weiss, Samuel and Cairncross, J Gregory and Phillips, Joanna J and Pieper, Russell O and Ronen, Sabrina M},
title = {{IDH1 Mutation Induces Reprogramming of Pyruvate Metabolism.}},
journal = {Cancer Research},
year = {2015},
volume = {75},
number = {15},
pages = {2999--3009},
month = aug,
publisher = {American Association for Cancer Research},
affiliation = {Department of Radiology and Biomedical Imaging, University of California San Francisco, San Francisco, California.},
doi = {10.1158/0008-5472.CAN-15-0840},
pmid = {26045167},
pmcid = {PMC4526330},
language = {English},
rating = {0},
date-added = {2018-03-16T12:39:55GMT},
date-modified = {2018-03-16T13:14:13GMT},
abstract = {Mutant isocitrate dehydrogenase 1 (IDH1) catalyzes the production of 2-hydroxyglutarate but also elicits additional metabolic changes. Levels of both glutamate and pyruvate dehydrogenase (PDH) activity have been shown to be affected in U87 glioblastoma cells or normal human astrocyte (NHA) cells expressing mutant IDH1, as compared with cells expressing wild-type IDH1. In this study, we show how these phenomena are linked through the effects of IDH1 mutation, which also reprograms pyruvate metabolism. Reduced PDH activity in U87 glioblastoma and NHA IDH1 mutant cells was associated with relative increases in PDH inhibitory phosphorylation, expression of pyruvate dehydrogenase kinase-3, and levels of hypoxia inducible factor-1$alpha$. PDH activity was monitored in these cells by hyperpolarized (13)C-magnetic resonance spectroscopy ((13)C-MRS), which revealed a reduction in metabolism of hyperpolarized 2-(13)C-pyruvate to 5-(13)C-glutamate, relative to cells expressing wild-type IDH1. (13)C-MRS also revealed a reduction in glucose flux to glutamate in IDH1 mutant cells. Notably, pharmacological activation of PDH by cell exposure to dichloroacetate (DCA) increased production of hyperpolarized 5-(13)C-glutamate in IDH1 mutant cells. Furthermore, DCA treatment also abrogated the clonogenic advantage conferred by IDH1 mutation. Using patient-derived mutant IDH1 neurosphere models, we showed that PDH activity was essential for cell proliferation. Taken together, our results established that the IDH1 mutation induces an MRS-detectable reprogramming of pyruvate metabolism, which is essential for cell proliferation and clonogenicity, with immediate therapeutic implications.},
url = {http://cancerres.aacrjournals.org/cgi/doi/10.1158/0008-5472.CAN-15-0840},
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uri = {url{papers3://publication/doi/10.1158/0008-5472.CAN-15-0840}}
}