by Izquierdo-Garcia, José Luis, Nin, Nicolás, Cardinal-Fernández, Pablo, Rojas, Yeny, de Paula, Marta, Granados, Rosario, Martínez-Caro, Leticia, Ruiz-Cabello, Jesus and Lorente, José Angel
Abstract:
The aim of this study is the identification of metabolomic biomarkers of sepsis and sepsis-induced acute kidney injury (AKI) in an experimental model. Pigs were anesthetized and monitored to measure mean arterial pressure (MAP), systemic blood flow (QT), mean pulmonary arterial pressure (MPAP), renal artery blood flow (QRA), renal cortical blood flow (QRC), and urine output (UO). Sepsis was induced at t=0 min by the administration of live Escherichia coli (n=6) or saline (n=8). At t=300 min, animals were sacrificed. Renal tissue, urine and serum samples were analyzed by Nuclear Magnetic Resonance (NMR) spectroscopy. Principal component analyses were performed on the processed NMR spectra to highlight kidney injury biomarkers. Sepsis was associated with decreased QT and MAP, and decreased QRA, QRC and urine output. Creatinine serum concentration and neutrophil gelatinase-associated lipocalin serum and urine concentrations increased. NMR-based metabolomics analysis found metabolic differences between control and septic animals: (i) in kidney tissue, increased lactate and nicotinuric acid, and decreased valine, aspartate, glucose and threonine; (ii) in urine, increased isovaleroglycine, aminoadipic acid, N-acetylglutamine, N-acetylaspartate and ascorbic acid, and decreased myoinositol and phenylacetylglycine; (iii) in serum, increased lactate, alanine, pyruvate and glutamine, and decreased valine, glucose and betaine concentrations. The concentration of several metabolites altered in renal tissue and urine samples from septic animals showed a significant correlation with markers of AKI (i.e., creatinine and NGAL serum concentrations). NMR-based metabolomics is a potentially useful tool for biomarker identification of sepsis-induced AKI.
Reference:
Identification of novel metabolomic biomarkers in an experimental model of septic acute kidney injury. (Izquierdo-Garcia, José Luis, Nin, Nicolás, Cardinal-Fernández, Pablo, Rojas, Yeny, de Paula, Marta, Granados, Rosario, Martínez-Caro, Leticia, Ruiz-Cabello, Jesus and Lorente, José Angel), In American journal of physiology. Renal physiology, volume 220, 2018.
Bibtex Entry:
@article{IzquierdoGarcia:2018kv,
author = {Izquierdo-Garcia, Jos{'e} Luis and Nin, Nicol{'a}s and Cardinal-Fern{'a}ndez, Pablo and Rojas, Yeny and de Paula, Marta and Granados, Rosario and Mart{'i}nez-Caro, Leticia and Ruiz-Cabello, Jesus and Lorente, Jos{'e} Angel},
title = {{Identification of novel metabolomic biomarkers in an experimental model of septic acute kidney injury.}},
journal = {American journal of physiology. Renal physiology},
year = {2018},
volume = {220},
pages = {284},
month = oct,
affiliation = {Molecular and Functional Biomarkers, CIC biomaGUNE, Spain.},
doi = {10.1152/ajprenal.00315.2018},
pmid = {30379100},
language = {English},
rating = {0},
date-added = {2018-11-06T11:51:33GMT},
date-modified = {2018-12-23T10:16:29GMT},
abstract = {The aim of this study is the identification of metabolomic biomarkers of sepsis and sepsis-induced acute kidney injury (AKI) in an experimental model. Pigs were anesthetized and monitored to measure mean arterial pressure (MAP), systemic blood flow (QT), mean pulmonary arterial pressure (MPAP), renal artery blood flow (QRA), renal cortical blood flow (QRC), and urine output (UO). Sepsis was induced at t=0 min by the administration of live Escherichia coli (n=6) or saline (n=8). At t=300 min, animals were sacrificed. Renal tissue, urine and serum samples were analyzed by Nuclear Magnetic Resonance (NMR) spectroscopy. Principal component analyses were performed on the processed NMR spectra to highlight kidney injury biomarkers. Sepsis was associated with decreased QT and MAP, and decreased QRA, QRC and urine output. Creatinine serum concentration and neutrophil gelatinase-associated lipocalin serum and urine concentrations increased. NMR-based metabolomics analysis found metabolic differences between control and septic animals: (i) in kidney tissue, increased lactate and nicotinuric acid, and decreased valine, aspartate, glucose and threonine; (ii) in urine, increased isovaleroglycine, aminoadipic acid, N-acetylglutamine, N-acetylaspartate and ascorbic acid, and decreased myoinositol and phenylacetylglycine; (iii) in serum, increased lactate, alanine, pyruvate and glutamine, and decreased valine, glucose and betaine concentrations. The concentration of several metabolites altered in renal tissue and urine samples from septic animals showed a significant correlation with markers of AKI (i.e., creatinine and NGAL serum concentrations). NMR-based metabolomics is a potentially useful tool for biomarker identification of sepsis-induced AKI.},
url = {https://www.physiology.org/doi/10.1152/ajprenal.00315.2018},
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