by Lechuga-Vieco, Ana V, Groult, Hugo, Pellico, Juan, Mateo, Jesus, Enríquez, Jose A, Ruiz-Cabello, Jesus and Herranz, Fernando
Abstract:
ApoB-100 and Phosphatidylcholine-specific phospholipase C (PC-PLC) are important contributors to atherosclerosis development. ApoB-100 is the main structural protein of LDL, being directly associated with atherosclerosis plaque generation. PC-PLC is highly expressed in atherosclerosis lesions and contributes to their progression. We show how phosphatidylcholine-coated nanomicelles can be used for specific characterisation of atherosclerosis plaque. Results show that ApoB-100 in the protein corona of the nanomicelle targets the particles to atherosclerotic areas in apolipoprotein E-/-mice. Furthermore, PC-PLC selectively removes the polar heads from the phospholipid coating of the nanomicelles leading to their accumulation. To fully characterise the behaviour of the nanomicelles, we developed multimodal probes using a nanoemulsion step. Hybrid imaging revealed plaque accumulation of the nanomicelles and colocalisation with PC-PLC expression and ApoB-100 in the plaque. This study shows how protein corona composition and enzyme-driven nanomaterial accumulation can be used for detection of atherosclerosis.
Reference:
Protein corona and phospholipase activity drive selective accumulation of nanomicelles in atherosclerotic plaques. (Lechuga-Vieco, Ana V, Groult, Hugo, Pellico, Juan, Mateo, Jesus, Enríquez, Jose A, Ruiz-Cabello, Jesus and Herranz, Fernando), In Nanomedicine: Nanotechnology, Biology and Medicine, volume 14, 2018.
Bibtex Entry:
@article{LechugaVieco:2018dkb,
author = {Lechuga-Vieco, Ana V and Groult, Hugo and Pellico, Juan and Mateo, Jesus and Enr{'i}quez, Jose A and Ruiz-Cabello, Jesus and Herranz, Fernando},
title = {{Protein corona and phospholipase activity drive selective accumulation of nanomicelles in atherosclerotic plaques.}},
journal = {Nanomedicine: Nanotechnology, Biology and Medicine},
year = {2018},
volume = {14},
number = {3},
pages = {643--650},
month = jan,
affiliation = {Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC) and CIBERES, Madrid, Spain.},
doi = {10.1016/j.nano.2017.12.021},
pmid = {29317346},
language = {English},
rating = {0},
date-added = {2018-03-16T12:48:35GMT},
date-modified = {2019-02-04T11:04:47GMT},
abstract = {ApoB-100 and Phosphatidylcholine-specific phospholipase C (PC-PLC) are important contributors to atherosclerosis development. ApoB-100 is the main structural protein of LDL, being directly associated with atherosclerosis plaque generation. PC-PLC is highly expressed in atherosclerosis lesions and contributes to their progression. We show how phosphatidylcholine-coated nanomicelles can be used for specific characterisation of atherosclerosis plaque. Results show that ApoB-100 in the protein corona of the nanomicelle targets the particles to atherosclerotic areas in apolipoprotein E-/-mice. Furthermore, PC-PLC selectively removes the polar heads from the phospholipid coating of the nanomicelles leading to their accumulation. To fully characterise the behaviour of the nanomicelles, we developed multimodal probes using a nanoemulsion step. Hybrid imaging revealed plaque accumulation of the nanomicelles and colocalisation with PC-PLC expression and ApoB-100 in the plaque. This study shows how protein corona composition and enzyme-driven nanomaterial accumulation can be used for detection of atherosclerosis.},
url = {http://linkinghub.elsevier.com/retrieve/pii/S1549963418300066},
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uri = {url{papers3://publication/doi/10.1016/j.nano.2017.12.021}}
}