by López-Melgar, Beatriz, Fernández-Friera, Leticia, Sánchez-González, Javier, Vilchez, Jean Paul, Cecconi, Alberto, Mateo, Jesus, Peñalvo, José L, Oliva, Belén, García-Ruiz, José M, Kauffman, Steve, Jimenez-Borreguero, Luis Jesús, Ruiz-Cabello, Jesus, Fernández-Ortiz, Antonio, Ibáñez, Borja and Fuster, Valentin
Abstract:
INTRODUCTION:Direct quantification of atherosclerotic plaque volume by three-dimensional vascular ultrasound (3DVUS) is more reproducible than 2DUS-based three-dimensional (2D/3D) techniques that generate pseudo-3D volumes from summed 2D plaque areas; however, its accuracy has not been reported. We aimed to determine 3DVUS accuracy for plaque volume measurement with special emphasis on small plaques (a hallmark of early atherosclerosis). METHODS:The in vitro study consisted of nine phantoms of different volumes (small and medium-large) embedded at variable distances from the surface (superficial vs. >5 cm-depth) and comparison of 3DVUS data generated using a novel volumetric-linear array method with the real phantom volumes. The in vivo study was undertaken in a rabbit model of atherosclerosis in which 3DVUS and 2D/3D volume measurements were correlated against gold-standard histological measurements. RESULTS:In the in vitro setting, there was a strong correlation between 3DVUS measures and real phantom volume both for small (3.0-64.5 mm(3) size) and medium-large (91.1-965.5 mm(3) size) phantoms embedded superficially, with intraclass correlation coefficients (ICC) of 0.99 and 0.98, respectively; conversely, when phantoms were placed at >5 cm, the correlation was only moderate (ICC = 0.67). In the in vivo setting there was strong correlation between 3DVUS-measured plaque volumes and the histological gold-standard (ICC = 0.99 [4.02-92.5 mm(3) size]). Conversely, the correlation between 2D/3D values and the histological gold standard (sum of plaque areas) was weaker (ICC = 0.87 [49-520 mm(2) size]), with large dispersion of the differences between measurements in Bland-Altman plots (mean error, 79.2 mm(2)). CONCLUSIONS:3DVUS using the volumetric-linear array method accurately measures plaque volumes, including those of small plaques. Measurements are more accurate for superficial arterial territories than for deep territories.
Reference:
Accurate quantification of atherosclerotic plaque volume by 3D vascular ultrasound using the volumetric linear array method. (López-Melgar, Beatriz, Fernández-Friera, Leticia, Sánchez-González, Javier, Vilchez, Jean Paul, Cecconi, Alberto, Mateo, Jesus, Peñalvo, José L, Oliva, Belén, García-Ruiz, José M, Kauffman, Steve, Jimenez-Borreguero, Luis Jesús, Ruiz-Cabello, Jesus, Fernández-Ortiz, Antonio, Ibáñez, Borja and Fuster, Valentin), In Atherosclerosis, volume 248, 2016.
Bibtex Entry:
@article{LopezMelgar:2016kuc,
author = {L{'o}pez-Melgar, Beatriz and Fern{'a}ndez-Friera, Leticia and S{'a}nchez-Gonz{'a}lez, Javier and Vilchez, Jean Paul and Cecconi, Alberto and Mateo, Jesus and Pe{~n}alvo, Jos{'e} L and Oliva, Bel{'e}n and Garc{'i}a-Ruiz, Jos{'e} M and Kauffman, Steve and Jimenez-Borreguero, Luis Jes{'u}s and Ruiz-Cabello, Jesus and Fern{'a}ndez-Ortiz, Antonio and Ib{'a}{~n}ez, Borja and Fuster, Valentin},
title = {{Accurate quantification of atherosclerotic plaque volume by 3D vascular ultrasound using the volumetric linear array method.}},
journal = {Atherosclerosis},
year = {2016},
volume = {248},
pages = {230--237},
month = may,
affiliation = {Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), 3 Melchor Fern{'a}ndez Almagro St, 28029 Madrid, Spain; Hospital Universitario HM Montepr{'i}ncipe, 25 Montepr{'i}ncipe Ave, 28660 Boadilla del Monte, Spain. Electronic address: beatriz.lopez@cnic.es.},
doi = {10.1016/j.atherosclerosis.2016.03.002},
pmid = {27038420},
language = {English},
rating = {0},
date-added = {2019-09-24T15:33:09GMT},
date-modified = {2019-10-09T09:21:58GMT},
abstract = {INTRODUCTION:Direct quantification of atherosclerotic plaque volume by three-dimensional vascular ultrasound (3DVUS) is more reproducible than 2DUS-based three-dimensional (2D/3D) techniques that generate pseudo-3D volumes from summed 2D plaque areas; however, its accuracy has not been reported. We aimed to determine 3DVUS accuracy for plaque volume measurement with special emphasis on small plaques (a hallmark of early atherosclerosis).

METHODS:The in~vitro study consisted of nine phantoms of different volumes (small and medium-large) embedded at variable distances from the surface (superficial vs. >5~cm-depth) and comparison of 3DVUS data generated using a novel volumetric-linear array method with the real phantom volumes. The in~vivo study was undertaken in a rabbit model of atherosclerosis in which 3DVUS and 2D/3D volume measurements were correlated against gold-standard histological measurements.

RESULTS:In the in~vitro setting, there was a strong correlation between 3DVUS measures and real phantom volume both for small (3.0-64.5~mm(3) size) and medium-large (91.1-965.5~mm(3) size) phantoms embedded superficially, with intraclass correlation coefficients (ICC) of 0.99 and 0.98, respectively; conversely, when phantoms were placed at >5~cm, the correlation was only moderate (ICC~=~0.67). In the in~vivo setting there was strong correlation between 3DVUS-measured plaque volumes and the histological gold-standard (ICC~=~0.99 [4.02-92.5~mm(3) size]). Conversely, the correlation between 2D/3D values and the histological gold standard (sum of plaque areas) was weaker (ICC~=~0.87 [49-520~mm(2) size]), with large dispersion of the differences between measurements in Bland-Altman plots (mean error, 79.2~mm(2)).

CONCLUSIONS:3DVUS using the volumetric-linear array method accurately measures plaque volumes, including those of small plaques. Measurements are more accurate for superficial arterial territories than for deep territories.},
url = {https://linkinghub.elsevier.com/retrieve/pii/S0021915016300776},
uri = {url{papers3://publication/doi/10.1016/j.atherosclerosis.2016.03.002}}
}