by Luxán, Guillermo, Casanova, Jesús C, Martínez-Poveda, Beatriz, Prados, Belén, D’Amato, Gaetano, MacGrogan, Donal, Gonzalez-Rajal, Alvaro, Dobarro, David, Torroja, Carlos, Martinez, Fernando, Izquierdo-Garcia, José Luis, Fernández-Friera, Leticia, Sabater-Molina, María, Kong, Young-Y, Pizarro, Gonzalo, Ibáñez, Borja, Medrano, Constancio, García-Pavía, Pablo, Gimeno, Juan R, Monserrat, Lorenzo, Jiménez-Borreguero, Luis J and de la Pompa, José Luis
Abstract:
Left ventricular noncompaction (LVNC) causes prominent ventricular trabeculations and reduces cardiac systolic function. The clinical presentation of LVNC ranges from asymptomatic to heart failure. We show that germline mutations in human MIB1 (mindbomb homolog 1), which encodes an E3 ubiquitin ligase that promotes endocytosis of the NOTCH ligands DELTA and JAGGED, cause LVNC in autosomal-dominant pedigrees, with affected individuals showing reduced NOTCH1 activity and reduced expression of target genes. Functional studies in cells and zebrafish embryos and in silico modeling indicate that MIB1 functions as a dimer, which is disrupted by the human mutations. Targeted inactivation of Mib1 in mouse myocardium causes LVNC, a phenotype mimicked by inactivation of myocardial Jagged1 or endocardial Notch1. Myocardial Mib1 mutants show reduced ventricular Notch1 activity, expansion of compact myocardium to proliferative, immature trabeculae and abnormal expression of cardiac development and disease genes. These results implicate NOTCH signaling in LVNC and indicate that MIB1 mutations arrest chamber myocardium development, preventing trabecular maturation and compaction.
Reference:
Mutations in the NOTCH pathway regulator MIB1 cause left ventricular noncompaction cardiomyopathy. (Luxán, Guillermo, Casanova, Jesús C, Martínez-Poveda, Beatriz, Prados, Belén, D’Amato, Gaetano, MacGrogan, Donal, Gonzalez-Rajal, Alvaro, Dobarro, David, Torroja, Carlos, Martinez, Fernando, Izquierdo-Garcia, José Luis, Fernández-Friera, Leticia, Sabater-Molina, María, Kong, Young-Y, Pizarro, Gonzalo, Ibáñez, Borja, Medrano, Constancio, García-Pavía, Pablo, Gimeno, Juan R, Monserrat, Lorenzo, Jiménez-Borreguero, Luis J and de la Pompa, José Luis), In Nature Medicine, volume 19, 2013.
Bibtex Entry:
@article{luxan_mutations_2013,
	title = {Mutations in the {NOTCH} pathway regulator {MIB1} cause left ventricular noncompaction cardiomyopathy.},
	volume = {19},
	url = {http://www.nature.com/articles/nm.3046},
	doi = {10.1038/nm.3046},
	abstract = {Left ventricular noncompaction (LVNC) causes prominent ventricular trabeculations and reduces cardiac systolic function. The clinical presentation of LVNC ranges from asymptomatic to heart failure. We show that germline mutations in human MIB1 (mindbomb homolog 1), which encodes an E3 ubiquitin ligase that promotes endocytosis of the NOTCH ligands DELTA and JAGGED, cause LVNC in autosomal-dominant pedigrees, with affected individuals showing reduced NOTCH1 activity and reduced expression of target genes. Functional studies in cells and zebrafish embryos and in silico modeling indicate that MIB1 functions as a dimer, which is disrupted by the human mutations. Targeted inactivation of Mib1 in mouse myocardium causes LVNC, a phenotype mimicked by inactivation of myocardial Jagged1 or endocardial Notch1. Myocardial Mib1 mutants show reduced ventricular Notch1 activity, expansion of compact myocardium to proliferative, immature trabeculae and abnormal expression of cardiac development and disease genes. These results implicate NOTCH signaling in LVNC and indicate that MIB1 mutations arrest chamber myocardium development, preventing trabecular maturation and compaction.},
	language = {English},
	number = {2},
	journal = {Nature Medicine},
	author = {Luxán, Guillermo and Casanova, Jesús C and Martínez-Poveda, Beatriz and Prados, Belén and D'Amato, Gaetano and MacGrogan, Donal and Gonzalez-Rajal, Alvaro and Dobarro, David and Torroja, Carlos and Martinez, Fernando and Izquierdo-Garcia, José Luis and Fernández-Friera, Leticia and Sabater-Molina, María and Kong, Young-Y and Pizarro, Gonzalo and Ibáñez, Borja and Medrano, Constancio and García-Pavía, Pablo and Gimeno, Juan R and Monserrat, Lorenzo and Jiménez-Borreguero, Luis J and de la Pompa, José Luis},
	month = feb,
	year = {2013},
	pmid = {23314057},
	note = {Publisher: Nature Publishing Group},
	pages = {193--201}
}