by Pellico, Juan, Fernandez-Barahona, Irene, Benito, Marina, Gaitán-Simón, Ángel, Gutiérrez, Lucía, Ruiz-Cabello, Jesus and Herranz, Fernando
Abstract:
The importance of atherosclerosis is driving research to create improved diagnostic tools based on molecular imaging. Pretargeted imaging is the use of bioorthogonal probes that selectively accumulate upon reaction with a pre-modified biomolecule in vivo. To date, this very promising approach has not been applied to atherosclerosis. Neither has been the use of a single nano-radiomaterial for PET / T1-MR imaging of atherosclerosis. Here, we synthesized bioorthogonal nano-radiomaterials for in vivo pretargeted molecular imaging in a mouse model of atherosclerosis. Based on tetrazine-ligation, these functionalized 68Ga iron oxide nano-radiomaterials provide simultaneous PET and T1-MRI signals and selectively accumulate in atherosclerotic plaques in mice sequentially injected with trans-cyclooctene-modified antibodies against oxidized LDL followed by the hybrid nano-radiomaterial. Our results demonstrate the ability of this approach to unambiguously detect atherosclerosis. Furthermore, we show the first example of how hybrid imaging can be used for pretargeted bioorthogonal molecular imaging with nanomaterials.
Reference:
Unambiguous detection of atherosclerosis using bioorthogonal nanomaterials. (Pellico, Juan, Fernandez-Barahona, Irene, Benito, Marina, Gaitán-Simón, Ángel, Gutiérrez, Lucía, Ruiz-Cabello, Jesus and Herranz, Fernando), In Nanomedicine: Nanotechnology, Biology and Medicine, volume 17, 2019.
Bibtex Entry:
@article{Pellico:2019fu,
author = {Pellico, Juan and Fernandez-Barahona, Irene and Benito, Marina and Gait{'a}n-Sim{'o}n, {'A}ngel and Guti{'e}rrez, Luc{'i}a and Ruiz-Cabello, Jesus and Herranz, Fernando},
title = {{Unambiguous detection of atherosclerosis using bioorthogonal nanomaterials.}},
journal = {Nanomedicine: Nanotechnology, Biology and Medicine},
year = {2019},
volume = {17},
pages = {26--35},
month = jan,
affiliation = {Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC) and CIBERES. C/ Melchor Fern{'a}ndez-Almagro 3. 28029, Madrid. Spain.},
doi = {10.1016/j.nano.2018.12.015},
pmid = {30654185},
language = {English},
rating = {0},
date-added = {2019-01-19T16:49:55GMT},
date-modified = {2019-03-05T15:29:00GMT},
abstract = {The importance of atherosclerosis is driving research to create improved diagnostic tools based on molecular imaging. Pretargeted imaging is the use of bioorthogonal probes that selectively accumulate upon reaction with a pre-modified biomolecule in vivo. To date, this very promising approach has not been applied to atherosclerosis. Neither has been the use of a single nano-radiomaterial for PET / T1-MR imaging of atherosclerosis. Here, we synthesized bioorthogonal nano-radiomaterials for in vivo pretargeted molecular imaging in a mouse model of atherosclerosis. Based on tetrazine-ligation, these functionalized 68Ga iron oxide nano-radiomaterials provide simultaneous PET and T1-MRI signals and selectively accumulate in atherosclerotic plaques in mice sequentially injected with trans-cyclooctene-modified antibodies against oxidized LDL followed by the hybrid nano-radiomaterial. Our results demonstrate the ability of this approach to unambiguously detect atherosclerosis. Furthermore, we show the first example of how hybrid imaging can be used for pretargeted bioorthogonal molecular imaging with nanomaterials.},
url = {https://linkinghub.elsevier.com/retrieve/pii/S1549963419300085},
local-url = {file://localhost/Users/jruizcabello/Documents/JRC/biblio/Papers-folder/Library.papers3/Files/61/61FEE6E3-EC69-4621-BBA4-676DB9FBBD3C.pdf},
file = {{61FEE6E3-EC69-4621-BBA4-676DB9FBBD3C.pdf:/Users/jruizcabello/Documents/JRC/biblio/Papers-folder/Library.papers3/Files/61/61FEE6E3-EC69-4621-BBA4-676DB9FBBD3C.pdf:application/pdf}},
uri = {url{papers3://publication/doi/10.1016/j.nano.2018.12.015}}
}