by Velasco, Carlos, Mota-Cobian, Adriana, Mota, Ruben A, Pellico, Juan, Herranz, Fernando, Galán-Arriola, Carlos, Ibáñez, Borja, Ruiz-Cabello, Jesus, Mateo, Jesus and España, Samuel
Abstract:
BACKGROUND:Here we evaluated the feasibility of PET with Gallium-68 (68Ga)-labeled DOTA for non-invasive assessment of myocardial blood flow (MBF) and extracellular volume fraction (ECV) in a pig model of myocardial infarction. We also aimed to validate MBF measurements using microspheres as a gold standard in healthy pigs. METHODS:8 healthy pigs underwent three sequential 68Ga-DOTA-PET/CT scans at rest and during pharmacological stress with simultaneous injection of fluorescent microspheres to validate MBF measurements. Myocardial infarction was induced in 5 additional pigs, which underwent 68Ga-DOTA-PET/CT examinations 7-days after reperfusion. Dynamic PET images were reconstructed and fitted to obtain MBF and ECV parametric maps. RESULTS:MBF assessed with 68Ga-DOTA-PET showed good correlation (y = 0.96x + 0.11, r = 0.91) with that measured with microspheres. MBF values obtained with 68Ga-DOTA-PET in the infarcted area (LAD, left anterior descendant) were significantly reduced in comparison to remote ones LCX (left circumflex artery, P < 0.0001) and RCA (right coronary artery, P < 0.0001). ECV increased in the infarcted area (P < 0.0001). CONCLUSION:68Ga-DOTA-PET allowed non-invasive assessment of MBF and ECV in pigs with myocardial infarction and under rest-stress conditions. This technique could provide wide access to quantitative measurement of both MBF and ECV with PET imaging.
Reference:
Quantitative assessment of myocardial blood flow and extracellular volume fraction using 68Ga-DOTA-PET: A feasibility and validation study in large animals. (Velasco, Carlos, Mota-Cobian, Adriana, Mota, Ruben A, Pellico, Juan, Herranz, Fernando, Galán-Arriola, Carlos, Ibáñez, Borja, Ruiz-Cabello, Jesus, Mateo, Jesus and España, Samuel), In Journal of nuclear cardiology : official publication of the American Society of Nuclear Cardiology, volume 3, 2019.
Bibtex Entry:
@article{Velasco:2019iz,
author = {Velasco, Carlos and Mota-Cobian, Adriana and Mota, Ruben A and Pellico, Juan and Herranz, Fernando and Gal{'a}n-Arriola, Carlos and Ib{'a}{~n}ez, Borja and Ruiz-Cabello, Jesus and Mateo, Jesus and Espa{~n}a, Samuel},
title = {{Quantitative assessment of myocardial blood flow and extracellular volume fraction using 68Ga-DOTA-PET: A feasibility and validation study in large animals.}},
journal = {Journal of nuclear cardiology : official publication of the American Society of Nuclear Cardiology},
year = {2019},
volume = {3},
pages = {623},
month = mar,
affiliation = {Centro Nacional de Investigaciones Cardiovasculares (CNIC), Melchor Fern{'a}ndez Almagro, 3, 28029, Madrid, Spain.},
doi = {10.1007/s12350-019-01694-z},
pmid = {30927149},
language = {English},
rating = {0},
date-added = {2019-04-01T07:55:13GMT},
date-modified = {2019-04-01T08:34:06GMT},
abstract = {BACKGROUND:Here we evaluated the feasibility of PET with Gallium-68 (68Ga)-labeled DOTA for non-invasive assessment of myocardial blood flow (MBF) and extracellular volume fraction (ECV) in a pig model of myocardial infarction. We also aimed to validate MBF measurements using microspheres as a gold standard in healthy pigs.

METHODS:8 healthy pigs underwent three sequential 68Ga-DOTA-PET/CT scans at rest and during pharmacological stress with simultaneous injection of fluorescent microspheres to validate MBF measurements. Myocardial infarction was induced in 5 additional pigs, which underwent 68Ga-DOTA-PET/CT examinations 7-days after reperfusion. Dynamic PET images were reconstructed and fitted to obtain MBF and ECV parametric maps.

RESULTS:MBF assessed with 68Ga-DOTA-PET showed good correlation (y = 0.96x + 0.11, r = 0.91) with that measured with microspheres. MBF values obtained with 68Ga-DOTA-PET in the infarcted area (LAD, left anterior descendant) were significantly reduced in comparison to remote ones LCX (left circumflex artery, P < 0.0001) and RCA (right coronary artery, P < 0.0001). ECV increased in the infarcted area (P < 0.0001).

CONCLUSION:68Ga-DOTA-PET allowed non-invasive assessment of MBF and ECV in pigs with myocardial infarction and under rest-stress conditions. This technique could provide wide access to quantitative measurement of both MBF and ECV with PET imaging.},
url = {http://link.springer.com/10.1007/s12350-019-01694-z},
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uri = {url{papers3://publication/doi/10.1007/s12350-019-01694-z}}
}